Journal: Journal of Translational Medicine

Article Title: The HKDC1-ASS1-ACSBG2 axis reprograms lipid metabolism to drive therapeutic resistance in hepatocellular carcinoma

doi: 10.1186/s12967-026-07779-x

Figure Lengend Snippet: Modulation of HKDC1 expression alters HCC proliferation in vitro. A ) Western blot analysis confirmed HKDC1 expression levels in MHCC97H cells transfected with an HKDC1-overexpressing plasmid and Hep3B cells transfected with shRNA targeting HKDC1 (sh-HKDC1#1, sh-HKDC1#2). B ) CCK-8 assays demonstrated differences in cell proliferation between HKDC1-modulated groups and controls. C ) EdU staining revealed variations in DNA replication activity across experimental groups. Scale bars: 50 μm. D ) Colony formation assays showed quantitative differences in colony numbers following HKDC1 expression modulation. E) Apoptosis assays detected altered apoptotic rates in Hep3B and MHCC97H cells upon HKDC1 knockdown or overexpression. F ) Subcutaneous xenograft tumors derived from HKDC1-modulated MHCC97H and Hep3B cells exhibited differential growth patterns over 28 days. G ) H&E, Ki67, and HKDC1 IHC staining in xenograft tumors. Scale bars: 50 μm. Data are presented as mean ± SD

Article Snippet: Cell apoptosis was quantified using a FITC Annexin V Apoptosis Detection Kit (Vazyme).

Techniques: Expressing, In Vitro, Western Blot, Transfection, Plasmid Preparation, shRNA, CCK-8 Assay, Staining, Activity Assay, Knockdown, Over Expression, Derivative Assay, Immunohistochemistry

Journal: Journal of Translational Medicine

Article Title: The HKDC1-ASS1-ACSBG2 axis reprograms lipid metabolism to drive therapeutic resistance in hepatocellular carcinoma

doi: 10.1186/s12967-026-07779-x

Figure Lengend Snippet: HKDC1 expression status correlates with therapeutic response in HCC models. A ) Dose-response assays revealed differential IC50 values of lenvatinib between HKDC1-modulated HCC cells (knockdown vs. overexpression) and controls. The concentration of Lenvatinib treated with cells are ste as: 0.01, 0.1 0.5, 1, 2, 4, 10, 20µM. B ) Apoptosis assays demonstrated decreased apoptotic rates in HKDC1-overexpression cells upon lenvatinib treatment. C ) Colony formation assays quantified reduced clonogenic survival under lenvatinib exposure in HKDC1-overexpression groups. D ) In vivo xenograft models showed weakened tumor growth suppression by lenvatinib in mice implanted with HKDC1- overexpression HCC cells. (Dose: 10 mg/kg/d for 28 days.) E ) Weight and volume growth curve of the xenograft tumors. F ) The comparable body weight gain across all groups demonstrates the absence of off-target toxicity. G ) H&E staining of necrosis and Nile red staining in xenograft tumors. Scale bars: HE: 50 μm; Nile red: 100 μm. Data are presented as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: Cell apoptosis was quantified using a FITC Annexin V Apoptosis Detection Kit (Vazyme).

Techniques: Expressing, Clinical Proteomics, Knockdown, Over Expression, Concentration Assay, In Vivo, Staining

Journal: Oncology Letters

Article Title: Effect of microRNA-27b on cisplatin chemotherapy sensitivity of oral squamous cell carcinoma via FZD7 signaling pathway

doi: 10.3892/ol.2019.10347

Figure Lengend Snippet: Effect of miR-27b overexpression on apoptosis of drug-resistant OSCC cells. Cisplatin-resistant cell group: Drug resistance group, DRG; Cisplatin resistance + miR-27b mimic group: Drug resistance + miR-27b mimic group, DRG+miR-27b mimic. *P<0.05; compared with DRG. OSCC, oral squamous cell carcinoma.

Article Snippet: The apoptosis rate was measured according to the procedure of the apoptosis detection kit Annexin V-FITC PI (Biotime, Shanghai, China).

Techniques: Over Expression

Journal: Translational Cancer Research

Article Title: Exploring the therapeutic potential of simvastatin in pancreatic neuroendocrine neoplasms: insights into cell cycle regulation and apoptosis

doi: 10.21037/tcr-24-363

Figure Lengend Snippet: SIM induces accumulation of G1 phase in QGP-1 cells and apoptosis in BON-1 cells. (A,B) Flow cytometry analysis of Cell-cycle distribution in both cell lines. (C,D) Flow cytometry analysis of the apoptotic cells using Annexin V/PI double-staining assay. (E,F) Western blot analysis of the protein expression of cell-cycle and apoptotic regulators. ns, P>0.05; *, P<0.05; **, P<0.01. NC, negative control; SIM, simvastatin; PI, propidium iodide.

Article Snippet: Apoptosis assay was detected using the Annexin V-FITC/PI Apoptosis Detection Kit A211 (APExBIO, Houston, USA).

Techniques: Flow Cytometry, Double Staining, Western Blot, Expressing, Negative Control